The vast repertoire of the chamber mixed choir ROSA is based upon a capella works of Renaissance composers. An important part of it is  represented by sacred music of all periods , Orthodox included. Pieces with instrumental accompaniment and  works of outstanding contemporary Czech composers were also added.
On the list of composers in the repertoire there are names such as Kryštof Harant z Polžic a Bezdružic, J. D. Zelenka, F. Tůma, Jakob Arcadelt, Gesualdo da Venosa, O. di Lasso, B. Trombocino, B. M. Černohorský, I. Cherubini, W. A. Mozart,. J. Brahms, A. Bruckner, P. Certon, Cl. Debussy, Fr. Poulenc, M. I. Glinka, G. Verdi, A. Vivaldi, J. Rossini, A. Dvořák, J. Busto, B. Martinů, Antonín Tučapský and Jaroslav Krček , not forgetting contemporary composers Zdeněk Lukáš and Eduard Douša.